无锡地区654例急性呼吸道病毒感染住院患儿的病原学研究

A study on viral pathogen of acute respiratory infection in 654 hospitalized children

目的探讨无锡地区儿童急性呼吸道病毒感染的病原学特点。方法采集2006年12月～2007年11月因急性呼吸道感染而住院治疗患儿的鼻咽分泌物,用直接荧光法检测呼吸道合胞病毒(RSV)、腺病毒(ADV)、流感病毒A、B型(IFVA、B)、副流感病毒Ⅰ、Ⅱ、Ⅲ型(PIVⅠ、Ⅱ、Ⅲ)共7种病毒抗原。结果654例患儿鼻咽分泌物中病毒抗原检测阳性共266例,阳性率40.6%;所检病毒依次为RSV(60.9%,162/266)、PIVⅢ型(19.1%)、IFVA型(7.5%)、APV(7.5%)、PIVⅠ型(2.6%)和IFVB型(2.2%)。冬春季节病毒抗原阳性率与夏秋季相近,喘息性疾病患儿阳性率明显高于非喘息性疾病患儿(P<0.01),3岁以下患儿检出阳性率明显高于3岁以上患儿(P<0.01),男性高于女性(P<0.01)。结论无锡地区急性呼吸道感染住院患儿病毒病原体以RSV和副流感病毒Ⅲ型为主,喘息性疾病和婴幼儿感染率较高。

Objective To study the infection state of virus in hospitalized children suffered with acute respiratory infection in Wuxi district. Methods Nasopharyngeal secretion was obtained from 654 in-patient children diagnosed as acute respiratory infection, and respiratory syncytial virus（RSV）, adenovirus, para influenza virus Ⅰ, Ⅱ, Ⅲ and influenza virus A, B were detected by direct immunofuorescence asssay. Results Of 654 samples, 266 samples（40. 6%） were shown to be viral positive,of which,RSV accounted for 162（60. 9%）, para influenza virus Ⅲ 51 （19. 1%）, adenovirus and influenza virus A 20 （7. 5 %）, para influenza virus Ⅰ 7 （2. 6 %）, and influenza virus B 3 （2. 2 %）. There was a higher virus detection rate in those with wheezing or/and aged 0 to 3 years old or male. Conclusion RSV and para influenza virus Ⅲ are the most common viral pathogen in children hospitalized with acute respiratory infection in Wuxi district.