半边莲生物碱缓解肾性高血压大鼠的血管重塑

Chinensis lour alkaloids alleviates vascular remodeling in renal hypertensive rats

目的:探讨半边莲生物碱对肾性高血压大鼠主动脉血管重塑的影响。方法:制备两肾一夹(2K1C)肾性高血压大鼠模型,灌服半边莲生物碱或卡托普利8周,用放射免疫技术测定血浆肾素活性(plasma rennin activity,PRA)。用Weigert法染色并测量腹主动脉中膜厚度及中膜截面积等血管重塑形态学参数。分别用Masson三色法和免疫组化方法测腹主动脉总胶原蛋白含量和Ⅰ型胶原蛋白含量。结果:高血压大鼠与假手术组相比PRA明显升高(P<0.05),使用半边莲生物碱后PRA显著降低(P<0.05);卡托普利对PRA无明显影响。高血压大鼠中膜厚度、中膜厚度/血管内径、中膜面积和腹主动脉胶原含量与假手术组相比明显升高(P<0.05);半边莲和卡托普利均可使高血压组大鼠上述指标显著降低(P<0.05)。结论:肾性高血压大鼠存在血管重塑现象,半边莲生物碱能抑制胶原的表达、降低肾素活性,对缓解血管重塑有一定作用。

AIM： To investigate the effect of lobelia chinensis lour alkaloids （LCLAs） on vascular remodeling in renal hypertension. METHODS ： The Glodblatt renovascular hypertension model was induced in rats by two-kidney one clip method. The renin activity （PRA） in plasma was determined by radioimmunoassay 8 weeks after the treatments with LCLAs or catoprill. The parameters of vascular remodeling, including media thickness （ MT）, luminal internal diameter （LD）, ratio of MT/LD and ratio of media cross-sectional area to lumen area （MSCA/LA） were measured through the Weigert staining photos of the abdominal aorta. Expressions of collagen and collagen I were measured respectively by the methods of Masson staining and immunohistochemistry. RESULTS： The PRA was significantly increased in hypertensive rats compared with the sham rats （P 〈0. 05）. PRA was significantly reduced in LCLAs group compared to control group. No inhibitory effect on PRA was observed in captopril group. The MT, the ratio of MT/LD, MSCA and the expression of collagen on abdominal aorta were significantly increased in hypertensive rats compared with the sham rats （ P 〈 0. 05 ）. LCLAs and captopril reduced these parameters raised in hypertensive rats （P 〈 0. 05 ）. CONCLUSION： The vascular remodeling occurs in renal hypertensive rats. LCLA inhibits the synthesis of the collagen and reduces PRA, which may alleviate the vascular remodeling in hypertensive rats.