乳腺癌细胞株MCF-7中锌和锌转运体表达的关系

Relationship between zinc and zinc transporter expression in breast cancer MCF-7 cells

目的:通过ZnCl2和TPEN处理培养人乳腺癌细胞株MCF-7,观察高锌和低锌两种状态下锌转运体mRNA的表达情况。方法:0、50、100、150和200μmol/L的ZnCl2以及0、5、10和15μmol/L的TPEN分别处理培养MCF-7细胞12h,细胞存活率用噻唑蓝(MTT)方法检测;荧光锌离子探针Zinquin检测细胞内锌离子含量;RT-PCR方法检测锌转运体(ZnT)mRNA的表达。结果:ZnCl2(浓度为150μmol/L和200μmol/L时)以及TPEN对MCF-7细胞均有生长抑制作用。ZnCl2处理后的MCF-7细胞内锌离子含量显著升高,TPEN处理后的MCF-7细胞内锌离子含量显著降低。与对照细胞相比,ZnCl2处理的细胞ZnT-1mRNA的表达水平随着ZnCl2浓度增加而依次升高;TPEN处理的细胞ZnT-1mRNA表达水平则普遍降低;ZIP2和ZIP10mRNA的表达水平随TPEN浓度的增加而依次升高。结论:高锌促进人乳腺癌MCF-7细胞ZnT-1mRNA的表达;低锌抑制人乳腺癌MCF-7细胞ZnT-1mRNA表达,促进ZIP2和ZIP10mRNA的表达。

AIM： To study the changes of zinc transporter gene expression in MCF- 7 cell line exposed to ZnCI2 and TPEN. METHODS： Human breast cancer cell line MCF -7 was exposed to different concentrations of ZnCI2 （0, 50, 100, 150,200 μmol/L） and TPEN （0, 5, 10, 15 μmol/L）, respectively. Twelve hours later, the cell viability was measured by MTr and levels of zinc transporter mRNA by RT-PCR. Zinquin was used to estimate the intracellular zinc concentrations. RESULTS： MCF-7 cells viability rate was significantly decreased when exposed to ZnCl2 （with 150μmol/L and 200 μmol/L） and TPEN. The intracellular zinc concentration was significantly increased when exposed to ZnCl2 and decreased when exposed to TPEN. ZnT - 1 mRNA level was increased along with the increasing concentration of ZnCl2 but decreased when exposed to TPEN. The expressions of ZIP2 and ZIP10 were increased along with the increasing concentration of TPEN. CONCLUSION： ZnT - 1 gene expression is induced by zinc supplement and repressed by zinc deficiency. ZIP2 and ZIP10 gene expressions are induced by zinc deficiency.