腺病毒潜伏感染对大鼠肺泡上皮细胞氧化／抗氧化平衡的影响

Adenovirus latent infection enhances the oxidant/antioxidant imbalance in rat alveolar epithelial cells

目的:研究腺病毒潜伏感染对大鼠肺泡上皮细胞氧化/抗氧化平衡的影响。方法:构建稳定表达腺病毒E1A蛋白的大鼠肺泡上皮细胞,检测氧化剂刺激时GSH、MDA的含量变化和主要抗氧化酶SOD、CAT、GPx、GST以及γ-GCS酶活性的变化。结果:腺病毒E1A蛋白通过抑制大鼠肺泡上皮细胞内γ-GCS酶的活性,抑制了氧化应激时GSH的合成和GPx及GST酶的活性;腺病毒E1A蛋白下调了细胞在氧化应激后的恢复能力。结论:腺病毒潜伏感染扩大了大鼠肺泡上皮细胞氧化应激时的氧化/抗氧化失衡,而腺病毒E1A蛋白抑制γ-GCS酶的活性是其中的关键环节。

AIM： To observe the influence of adenovirus latent infection on the oxidant/antioxidant balance in rat alveolar epithelial cells. METHODS： The rat alveolar epithelial cells were stably transfected with the plasmid pE1Aneo and control plasmid pneo. GSH and MDA contents, the activities of major anti-oxidative enzymes including SOD, CAT, GPx, GST and γ- GCS were detected in oxidant stress. RESULTS： Adenovirus E1A expression repressed the activity of γ- GCS, and decreased GSH contents in oxidant stress. As a result, the activity of GPx and GST was decreased. The contents of MDA maintained high in oxidant stress. CONCLUSION： Adenovirus latent infection amplifies the oxidant/antioxidant imbalance in rat alveolar epithelial cells in oxidants stress, and adenovirus E1A protein decreases the activity of γ- GCS, which plays an important role in this process.