摘要
背景:陡脉冲可使肿瘤细胞膜发生不可逆性电击穿导致细胞死亡,目前已在细胞实验和体外实验中证明了陡脉冲杀伤肿瘤细胞的可行性。目的:基于陡脉冲理论,观察脉冲作用前后作用区域的即刻温度变化,以及陡脉冲作用后肿瘤组织的病理学变化变化,以验证陡脉冲电场的生物学效应。设计、时间及地点:对比观察实验,于2007-05/2008-03在重庆医科大学医学超声工程研究所完成。材料:新西兰大白兔30只,建立VX2移植性肝癌模型。VX2瘤株由重庆医科大学超声研究所提供,陡脉冲治疗仪由重庆大学输配电装备及系统安全与新技术国家重点实验室自行设计。方法:将模型兔根据施加电压不同随机分为500,600,700,800V电压组,每组6只,给予组合脉冲处理。电压分别500600,700,800V,脉宽均为10μs,频率为20Hz,持续10min。并设空白对照组,仅插入电极而不开通电源,不予脉冲处理。主要观察指标:监测陡脉冲作用前后同一部位肿瘤组织的温度变化;取作用后肿瘤组织送光镜、电镜检查。结果:30只兔均进入结果分析。陡脉冲电场覆盖区组织温度升高不明显,脉冲作用前后温度变化无统计学差别(P>0.05)。苏木精-伊红染色显示脉冲作用活体兔肝VX2肿瘤组织后引起肿瘤组织凝固性坏死,电镜显示肿瘤细胞受到不可逆性损伤结论:陡脉冲可致肿瘤细胞发生不可逆性损伤,其对组织的损伤是一种非热效应,并非传统意义上的热疗。
Steep pulse therapy can electrically breakdown tumor cell membrane and cause cell death. It is proved that the steep pulsed electric fields can kill tumor cell by in vivo and vitro trials. OBJECTIVE: To observe the immediate temperature changes in stimulation area and pathological change of tumor tissue induced after steep pulse to investigate the biological effects of steep pulse. DESIGN, TIME AND SETTING: The comparative observation experiment was performed at Institute of Ultrasound Engineering in Medicine from May 2007 to March 2008. MATERIALS: Thirty New Zealand white rabbits were used to establish models of VX2 liver tumor; VX2 was provided by Institute of Ultrasound Engineering in Medicine of Chongqing Medical University; steep pulse treatment device was designed and developed by the State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing University. METHODS: Rabbits with VX2 liver tumor were randomly divided into four treatment groups according to voltage (500, 600, 700, and 800 V) and control group (n =6). Every rabbit in the treatment groups was exposed to steep pulse electric field with 500, 600, 700, and 800 V, respectively, pulse width 10 μs, frequency 20 Hz for 10 minutes, The control group was not given pulse treatment but only with electrode insertion. MAIN OUTCOME MEASURES: Temperature changes in tumor tissue of the same site were monitored before and after steep pulse, and tumor tissues were harvested after pulse treatment and observed under light microscopy and electron microscopy. RESULTS: Thirty rabbits were all included in final analysis. No significant temperature rise was detected in tissues exposed to electric field (P 〉 0.05). Hematine-eosine staining showed that pulse treatment induced tumor tissue coagulation necrosis; electron microscopic observation suggested irreversible damage in tumor cells. CONCLUSION: Steed pulse causes an irreversible damage in tumor cells, which is a non-thermal effect.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第22期4201-4204,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金资助项目(50407007)~~