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人胶质瘤细胞株U-251及其诱导肿瘤干细胞对化疗药物敏感性的研究 被引量：1

Research on the Chemotherapeutic Sensitivity of U-251 Glioma Cells and Glioma Stem Cells
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摘要 目的观察替尼泊苷(VM-26)、依托泊苷(VP-16)及氟尿嘧啶(5-FU)对U-251人胶质瘤细胞及U-251胶质瘤干细胞的生长抑制作用,探讨胶质瘤干细胞对传统化疗药物敏感性.方法运用无血清培养法从人胶质瘤细胞株U-251中培养分离出胶质瘤干细胞,然后用MTT比色法测定氟尿嘧啶、依托泊苷、替尼泊苷对U-251胶质瘤细胞及U-251胶质瘤干细胞的增殖抑制作用,比较U-251胶质瘤细胞及U-251胶质瘤干细胞对这3种化疗药物的敏感性.结果U-251胶质瘤细胞对血浆峰浓度值的VM-26、VP-16和5-FU的敏感性明显高于U-251胶质瘤干细胞.结论对U-251胶质瘤的生长起关键作用的U-251胶质瘤干细胞,对血浆峰浓度值下的VM-26、VP-16和5-FU不敏感,胶质瘤的治疗应靶向胶质瘤干细胞. Objective To study the sensitivity of U -251 glioma cells and glioma stem cells to some chemotherapeutic drugs （Vm -26, VP -16, 5 -FU） , so as to provide some effective clues for clinic chemotherapy. Methods Glioma stem cells were isolated from U - 251 human glioma cell line, MTT assay was performed to evaluate the inhibition effects upon the proliferation of U -251 glioma cells and glioma stem cells, and the sensibility of these drugs were compared. Result The inhibition rate of VM - 26, VP - 16, 5 - FU on glioma cells were evidently higher than glioma stem cells. Conclusion Glioma stem cells are more resistant to chemotherapy than glioma cells, the latter is the main target for chemotherapy.
作者 余化霖 肖宗宇 雷三喜 王宗宝 马以骝 董坚
机构地区 昆明医学院第一附属医院神经外科 昆明医学院第一附属医院生物治疗中心
出处 《昆明医学院学报》 2008年第3期22-27,共6页 Journal of Kunming Medical College
关键词 胶质瘤 脑肿瘤干细胞 化疗敏感性 Glioma Brain tumor stem cell Chemotherapy sensitivity
分类号 R730.53 [医药卫生—肿瘤]
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参考文献13

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同被引文献24

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昆明医学院学报
2008年 第3期

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