胰岛素样生长因子-Ⅱ对小鼠早期胚胎体外发育的影响

Effect of IGF-Ⅱ on Development of Mouse Preimplantation Embryo in Vitro

目的探讨胰岛素样生长因子-Ⅱ(IGF-Ⅱ)对小鼠早期胚胎的影响,为人类胚胎培养系统的优化提供依据.方法在mKSOM培养基中加入不同浓度的IGF-Ⅱ,对小鼠1-细胞胚胎进行体外培养,观察囊胚发育率、孵化率及胚胎细胞数的变化.结果小鼠1-细胞胚胎体外培养120h后,IGF-Ⅱ添加组囊胚发育率、孵化率显著高于对照组,但囊胚细胞计数与对照组无明显差别.结论添加IGF-Ⅱ对小鼠胚胎发育具有一定的促进作用.

Objective To evaluate the influence of insulin-like growth factor-Ⅱ （IGF-Ⅱ ） on the development of mouse preimplantation embryo in vitro. Methods One-cell mouse embryos were cultured for 120 hours in the mKSOM media supplemented with IGF- Ⅱ at different concentrations. The rate of development to blastocyst, hatched blastocyst and the cell number of each blastocyst were determined. Results IGF- Ⅱ caused a significant increase in the rate of blastocyst and hatched blastocyst, but no increase in the cell number of blastocyst was detected. Conclusion IGF- Ⅱ can promote the development of mouse preimplantation embryo in vitro.