地西泮对小鼠自主活动和学习记忆功能的影响

Effects of Diazepam on spontaneous motor activity and learning and memory function in mice

目的:观察不同剂量地西泮(Diazepam,D)对小鼠自主活动和学习记忆功能的影响。方法:昆明种小鼠120只,随机分成4组:生理盐水组(NS组),地西泮0.5mg/kg组(D0.5组),地西泮1.0mg/kg组(D1.0组),地西泮2.0mg/kg组(D2.0组),每组10只。观察不同剂量地西泮对小鼠自主活动计数、跳台法(step-down test)和避暗法(step-through test)的潜伏期和错误次数的影响。结果:与NS组比较,地西泮可剂量依赖性地减少小鼠自主活动次数计数,缩短跳台法和避暗法的潜伏期,增多错误次数。结论:地西泮能减少小鼠自主活动,抑制小鼠学习记忆功能,但在停药3d后此作用消失。

AIM： To study the effects of different doses of Diazepam on mice spontaneous motor activity, learning and memory function. METHODS： 120 Kunming mice were randomly divided into 4 groups. NS Group was given by hypodermic injection normal saline with the dose of 10 mL/kg; the other 3 groups（groups D0.s, Dr.0 and D2.0 ） were given by intraperitoneal injection diazepam with the different doses of 0.5 mg/kg, 1.0 mg/kg and 2.0 mg/kg respectively. The mice were then conducted to take step-down test and step-through test to evaluate the effects of Diazepam on learning and memory function by studying the latency of reaction and the number of errors in its performance. RESULTS： Compared with NS Group, Diazepam reduced the number of autonomic activities with dose-dependently, shortened the latency of reaction and increased the number of errors of step-down test and step-through test. CONCLUSION： Diazepam can decrease the mice spontaneous motor activity, restrain the learning and memory function of mice, and the effects disappear quickly after 3 days.