干扰素α对慢性髓系白血病来源的树突状细胞表达Fas/FasL的影响(英文)

Influence of Interferon α on Expression of Fas and Fas Ligand in Dendritic Cells from Patients with Chronic Myeloid Leukemia

本研究探讨干扰素α对慢性髓系白血病(CML)来源的树突状细胞(DC)表达Fas/FasL的影响。在CML-DCs的培养液中除加入SCF,GM-CSF,TNF-α及IL-4外,还加入IFN-α。培养10-14天,除了鉴定细胞免疫表型和Ph1染色体比例外,还应用流式细胞仪检测细胞表达Fas/FasL比例,用PI染色分析细胞凋亡,ELISA法检测上清液sFas含量。结果表明:加入IFN-α后,CML-DC共刺激分子的表达显著改善,Ph1(+)细胞比例随IFN-α浓度增加而减低;培养细胞Fas的表达上调,sFas含量却下降,FasL表达阴性,细胞凋亡比例增加。结论:IFN-α在改善CML-DC表型同时,可通过Fas途径促进Ph1(+)细胞凋亡,使Ph1(-)细胞数量相对增加。

The study was aimed to investigate the influence of interferon α （IFN-α） on the expressions of Fas and Fas ligand （FasL） in dendritic cells （DCs） from patients with chronic myeloid leukemia （CML）. In addition to adding stem cell factor（ SCF）, granulocyte-macrophage colony-stimulating factor （GM-CSF）, tumor necrosis factor α （TNF- α） and interleukin 4 （ IL-4）, the IFN-α was added to the serum-free medium for DCs. After culturing for 10-14 days, cell phenotype and percentage of Ph1 chromosome were detected by different methods. The expression of Fas or FasL on CML-DCs and cell cycle of DCs labeled with propidium iodine （PI） were measured by flow cytometry. The concentra- tion of sFas in supernatants was analyzed by enzyme-linked immunosorbent assay （ELISA）. The results indicated that the expression of co-stimulatory molecules were improved significantly while the percentages of Ph1 positive cells decreased. The level of Fas on cells was up-regulated and the concentration of sFas decreased. However, the expression of FasL was negative. The ratio of apoptosis rose gradually while the concentration of IFN-α increased. It is concluded that IFN-α can accelerate the apoptosis of Ph1 positive cells through Fas/FasL pathway, so the number of Ph1 negative cells increases relatively.