龟板、黄芪、丹参和党参诱导K562细胞合成γ珠蛋白的实验研究

γ-globin Synthesis in K562 Cells Induced with Tortois Plastron,Astragali,Salviae Miltiorrhizae and Codonopsis Pilosulae

本研究探讨龟板、黄芪、丹参和党参对K562细胞γ-珠蛋白合成的影响。以K562细胞为体外模型,用联苯胺染色方法确定龟板、黄芪、丹参及党参4种中药诱导K562细胞血红蛋白表达的剂量效应和时间效应,并用Western blot技术检测血红蛋白F(α2γ2)水平。结果表明:龟板、黄芪、丹参和党参作用于K562细胞6天,联苯胺染色阳性率最高分别可达23.5%、19.8%、15.8%和14.5%;Western blot检测显示血红蛋白F表达增加。结论:龟板、黄芪、丹参及党参在体外可诱导K562细胞γ珠蛋白的合成水平增高,从而使血红蛋白F增加。4种中药具有与阳性对照丁酸钠相似的诱导γ珠蛋白合成增加的作用。

This study was purposed to investigate the effects of tortois plastron, astragali, salviae miltiorrhizae and codonopsis pilosulae on γ-globin gene synthesis in K562 cells in vitro. Benzidine staining was used to clarify the doseand time-dependent effects of tortois plastron, astragali, salviae miltiorrhizae and codonopsis pilosulae on hemoglobin synthesis in K562 cells and Western blotting was performed to determine the level of hemoglobin F（ α2γ2 ）. The resuts indicated that the K562 cells treated with 4 kinds of traditional Chinese medcine had different stain rates of benzidine for： 23.5% （ tortois plastron ） , 19.8% （ astragali ） , 15.8% （ salviae miltiorrhizae ） and 14.5% （ codonopsis pilosulae ） at 6 days after the treatment. Western blot indicated that synthesis of HbF increased. It is concluded that tortois plastron, astragali, salviae miltiorrhizae and codonopsis pilosulae enhance globins-γ synthesis level and increase hemoglobin F level in K562 cells , the effect of which resembles that of sodium butyrate.