摘要
目的制备3种季铵盐类单体,比较其对口腔常见细菌的作用,为牙科抗菌材料的研制提供基础依据。方法制备3种季铵盐类单体[甲基丙烯酰氧乙基.苄基-二甲基氯化铵( methacryloxylethyl benzyl dimethyl ammonium chloride, DMAE-BC)、甲基丙烯酰氧乙基-间氯苄基-二甲基氯化铵(methacryloxylethyl m-chloro benzyl dimethyl ammonium chloride, DMAE-m-CBC)、甲基丙烯酰氧乙基-正十六烷基-二甲基氯化铵(methacryloxylethyl cetyl dimethyl ammonium chloride, DMAE-CB)],采用液体稀释法比较其对变形链球菌、血链球菌、牙龈卟啉单胞菌、产黑普氏菌的抗菌作用。结果3种季铵盐类单体中DMAE-CB的抗菌效果最强,对4种口腔细菌的最低抑菌浓度值为1.2至4.8ms/L,而另外两种单体对4种细菌的最低抑菌浓度值为1562.5至3125.0mg/L。结论3种季铵盐类单体对变形链球菌、血链球菌、牙龈卟啉单胞菌、产黑普氏菌均有不同程度的抗菌作用,其中DMAE-CB的抗菌活性最强。
Objective To prepare three quaternary ammonium salt (QAS) monomers, and to compare their antibacterial activities against four oral bacterial strains. Methods Three antibacterial monomers[methacryloxylethyl benzyl dimethyl ammonium chloride( DMAE-BC ), methacryloxylethyl m-chloro benzyl dimethyl ammonium chloride( DMAE-m-CBC), methacryloxylethyl cetyl dimethyl ammonium chloride (DMAE-CB) ] were synthesized according to the general structure of target monomers. Their antibacterial effects were investigated using the broth dilution test on Gram-positive and Gram-negative bacterial strains ( Streptococcus mutans,Streptococcus sanguis, Porphyromonas gingivalis, Prevotella melaninogenica). Results Three different monomers were successfully obtained. All the tested bacterial strains were susceptible to the three monomers, among which DMAE-CB exhibited the lowest minimal inhibitory concentrations ( MIC ) ranging from 1.2 to 4. 8 mg/L. Conclusions All these three QAS monomers have different antibacterial activities against four oral bacteria strains. The data indicate that DMAE-CB may be a candidate antibacterial agent for oral infectious diseases.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2008年第6期370-373,共4页
Chinese Journal of Stomatology
基金
国家自然科学基金(30772444)
关键词
微生物敏感性试验
牙科材料
季铵盐
单体
Microbial sensitivity tests
Dental materials
Quaternary ammonium salt
Monomer