摘要
目的探讨肝素酶(heparanase)在胃癌组织中表达及其与胃癌临床病理特征之间的关系。方法采用免疫组织化学方法检测80例胃癌石蜡切片和20例正常胃黏膜中肝素酶的表达情况;分析其表达与胃癌患者年龄、性别、分期、分化程度、浸润深度、淋巴结转移的关系。结果80例胃癌中有49例(61%)呈肝素酶阳性表达,主要表达在癌细胞质和(或)细胞膜中,在正常胃黏膜上皮则呈阴性表达,肝素酶在胃癌中表达明显增高(P<0.05)。表达与胃癌的分化程度、分期、浸润深度、淋巴结转移有关,与患者年龄、性别无关。结论肝素酶在胃癌组织中的表达较正常胃黏膜上皮明显上调,其表达与胃癌的侵袭及转移能力密切相关。
Objective To assess protein expression levels of heparanase and the relation between their expression and biological factors in human gastric carcinomal Methods A total of 80 paraffin embedded gastric cancer and 20 normal specimens were studied by immunohistochemisty to evaluate the protein expression status of heparanase. The relation was analyzed among the protein expression and age, sex, TNM stage, cell differentiation, invasion depth, lymphatic metastasis. Results Immunohistochemistry showed that 49 specimens were heparanase positive among 80 cancer specimens. Human heparanase were highly expressed in cancer cell in contrast to none expression in normal tissue. Expression was in both cytoplasm and membrane. A significant correlation was found between the expression of either protein and cell differentiation, TNM stage, invasion depth, lymphatic metastasis. Conclusion Heparanase was highly expressed in colorectal cancer. It may play an important role in tumor invasion, metastasis and angiogenesis.
