IGF-Ⅱ和HBxAg及ras基因在树鼠句实验性肝癌组织中的表达

Expressions of IGFⅡ,HBxAg and ras Gene in the Experimental Hepatocellular Carcinomas in Tree Shrews

用免疫组化方法观察胰岛素样生长因子Ⅱ（ＩＧＦ－Ⅱ）、ＨＢｘＡｇ及ｒａｓ基因的表达蛋白ｐ２１在乙肝病毒（ＨＢＶ）和黄曲霉素Ｂ１（ＡＦＢ１）致树鼠句肝癌过程中的表达。发现这三种基因蛋白的表达与肝癌发生呈正相关系。即接受ＨＢＶ和ＡＦＢ１双因素的树鼠句的肝癌发生率与ＩＧＦ－Ⅱ、ＨＢｘＡｇ及ｐ２１的表达阳性率均显著高于只暴露于单一因素ＨＢＶ或ＡＦＢ１的动物。同时，在带瘤树鼠句中，这三种基因的表达又显著高于无瘤者。提示：ＨＢＶ与ＡＦＢ１能协同激发这些基因的异常表达；

The expressions of IGFⅡ(insulinlike growth factor Ⅱ),HBxAg(hepatitis B virus) and p21 were detected in the courses of hepatocarcinogenesis induced by HBV (hepatitis B virus) and/or AFB1 (aflatoxin B1) with immunohistochemical method.The parallel relations in the incidence of HCC (hepatocellular carcinoma) and the expressions of these genes have appeared in each group.They were higher in the tree shrews received both HBV and AFB1 than that exposed to HBV or AFB1 alone.On the other hand,the expressions of these genes in tumorcarried tree shrews were significantly higher than that in nontumor animals. Our results suggested that there was a synergistic effect of HBV and AFB1 in activation of these genes in tree shrews.Over expressions of these genes may take an important role in the courses of hepatocarcinogenesis in tree shrews.