大豆寡糖对瘤胃微生物区系的影响

Effects of Soybean-Oligosaccharids on Ruminal Microflora

本文旨在研究大豆寡糖对绵羊瘤胃细菌总数、纤维分解菌数、甲烷产生菌数和瘤胃原虫数的影响。选用9只带有永久性瘤胃瘘管的试验羊,随机分为3组,饲喂相同的基础日粮,并在此基础上分别灌注占干物质采食量(DMI)1.2%、0.6%和0.0%的大豆寡糖。细菌和纤维菌的计数采用Hungate的滚管法;甲烷菌的计数采用最大可能计数法进行;原虫计数采用血球计数室计数法。结果表明,灌注大豆寡糖提高了瘤胃纤维分解菌的数量,降低了甲烷产生菌的数量。这表明大豆寡糖对绵羊瘤胃微生物区系有着较为显著的影响,有利于中性洗涤纤维(NDF)在瘤胃内的降解和减少饲料能量的损失。

This paper aimed to study the effects of soybean-oligosaccharids on, the total number of sheep rumen bacteria, the number of cellulolytic bacteria, methanogen and the number of rumen protozoa. Nine sheep with permanent rumianl cannulas were randomly divided into three groups and each group was fed with the same basal diet. Based on the basal diet, the soybean-oligosaccharids accounting for 1.2% DMI, 0.6% DMI and 0.0% DMI was infused into each group respectively. The number of total bacterial and cellulolytic bacteria were counted by roll-tube technique of Hungate, the number of methanogen was counted by Maximum Probable Number, and the number of protozoa was counted by haemacytometer. The result showed that the number of rumen cellulolytic bacteria was increased by the infusion of soybean-oligosaccharids and the number of methanogen was decreased, which indicated that soybean-oligosaccharids had significant effect on the rumen microflora of the sheep and was favorable for the degradation of NDF in rumen and the reduction of the losses of feed energy.