高效液相色谱法测定紫丁香叶中酪醇的含量

To Establish a Method for the Determination of Tyrosol in Syring oblat A Lindl Leaves

目的：高效液相色谱法测定紫丁香叶中酪醇的含量。方法：采用HPLC法测定紫丁香叶中酪醇的含量。色谱柱：SymmetryC18柱（4．6mm×250mm，10μm）；流动相：甲醇-0．125％冰醋酸溶液（10：90）；流速：1．0ml／min；检测波长：275nm；柱温：室温。结果：酪醇在0．44—3．52μg范围内线性关系良好，回归方程为方程Y=616731x-9957．4，r=0．9993；平均回收率为97．83％。结论：该方法准确可靠，适用于紫丁香叶酪醇的含量测定。

Objective ： To establish a method for the determination of tyrosol in Syringa oblatA Lindl. Leaves. Methods ： HPLC was used on Symmetry C18 column （4.6mm × 250mm, 10um） ; with methand-aceticacid 0. 125% （10：90） as a mobile phase and 1 .0ml/min of flow rate. The detection wavelength was 275nm, and column temperature was room temperature. Results： The calibration curve of tyrosol was linear at the range of 0.44-3.52ug.The regression equation was y = 616731x -9957.4, r = 0.9993. The average recoveries were 98.01%. Condution ： The method is highly accurate and reliable, and is suitable for the determination of tyrosol in Syringa oblata Lindl. Leaves.