老年患者大肠埃希菌分离株对氯霉素耐药性及耐药相关基因研究

Chloramphenicol-resistant Escherichia coli and Its Resistance Genes Isolated Strains from Old Patients

目的了解老年患者耐氯霉素大肠埃希菌基因存在状况。方法采用聚合酶链反应及序列分析20株大肠埃希菌的catB和cmlA基因。结果catB和cmlA基因的阳性率均为20%,有2株大肠埃希菌catB和cmlA基因同时阳性。结论临床分离的耐氯霉素大肠埃希菌与产生耐药基因密切相关,在大肠埃希菌中检出catB和cmlA基因均为国内首次报道。

OBJECTIVE To investigate the chloramphenicol-resistant gene in Escherichia coli strains isolated from the old patients. METHODS Twenty strains of E. coli were clinically isolated. The resistance genes were analyzed by polymerase chain reaction（PCR） and verified by DNA sequencing. RESULTS The positive rates of catB and cmlA were 20~~0 and 20Y0, respectively. Two strains of E. coli were found containing catB gene and cmlA gene. CONCLUSIONS The resistance to chloramphenicol in these 20 strains of E, coli has a close relation to antimicrobial-resistance gene existed, and the genes of catB and cmlA in E. coli are all firstly found and reported in China.