手术切口感染的病原菌分布及耐药性探讨

Pathogen Distribution and Drug Resistance in Infection of Operative Incision

目的探讨手术切口感染病原菌的分布及耐药性,给临床防治手术切口感染提供科学依据。方法菌株分离鉴定按《全国临床检验操作规程》进行,药敏试验采用DADE BEHRING专用细菌鉴定药敏复合板。结果140株病原菌以革兰阴性杆菌为主,检出82株,占58.6%;革兰阳性球菌58株,占41.4%;其中金黄色葡萄球菌(SAU)和铜绿假单胞菌(PAE)检出率较高,分别为29.3%与27.1%;菌株均显示出较高的耐药性,其中耐甲氧西林金黄色葡萄球菌(MRSA)占SAU总数的31.7%;产超广谱β-内酰胺酶(ESBLs)大肠埃希菌和肺炎克雷伯菌检出率为40.9%。结论必须采取有效的防治措施,预防与控制手术切口感染。

OBJECTIVE To investigate pathogen distribution and drug resistance in infection of operative incision and provide the reference for clinical prevention and cure. METHODS Referring to National Rules of Operation in Clinical Laboratory, strains were isolated and identified, and drug-sensitivity test was performed by DADE BEHRING panel. RESULTS Among total 140 strains of pathogenic bacteria, the most were Gram-negative bacilli that accounted for 58.6% （82 strains）, followed by Gram-positive cocci that accounted for 41.4% （58 strains）. Staphylococcus aureus and Pseudornonas aeruginosa had the higher detection rate. The former was 29.3 % and the latter was 27. 1%. Among the total S. aureus, meticillin-resistant S, aureus （MRSA） accounted for 31. 7%. Detection rate of extended-spectrum beta-lactamases （ESBLs） producing Escherichia coli and Klebsiella pneurnoniae was 40.9%. CONCLUSIONS Effective measurement should be taken to prevent and control infection of operative incision.