暴发性肝衰竭小鼠肝组织胎球蛋白A的变化及其病理学意义

The expression of fetuin-A and its pathological significance in fulminent hepatic failure in mice

目的研究胎球蛋白A在暴发性肝衰竭（FHF）小鼠肝组织中的表达变化及其与肝组织损伤、炎症和肝细胞凋亡的关系。方法采用Western blot法、免疫组织化学法和逆转录聚合酶链反应法，分别检测暴发性肝衰竭小鼠肝组织中胎球蛋白A及其mRNA的表达变化；对组织损伤进行病理评分，采用免疫组织化学法检测肝组织中肿瘤坏死因子（TNF）α的表达，原位末端标记法检测其凋亡程度。结果在FHF小鼠中，胎球蛋白AmRNA和蛋白顷表达均明显下降，造模9h时改变最明显，吸光度比值分别为0．19±0．03和0．61±0．02，与正常组（0．82±0．01和1．12±0．13）比较，差异有统计学意义，P〈0．01；与肝组织病理评分和TNFα表达呈显著负相关。结论在FHF小鼠模型中，胎球蛋白A可能通过抑制TNFα等炎症因子来抑制肝组织炎症并减轻肝细胞凋亡，可能是一种肝损伤保护因子。

Objective To explore the dynamic changes of fetuin-A expression and the influences of the changes on liver damage, hepatocyte apoptosis and inflammation in a mouse fulminent hepatic failure （FHF） model. Methods The changes of fetuin-A expression were investigated by semi-quantitative RT-PCR and Western blot. lmmunohistochemical staining was used in TNF α and fetuin-A detection. Hepatocyte apoptosis was detected by TUNEL. Results Fetuin-A mRNA expression decreased after the FHF model was established for 3 hours （compared with the normal group, P 〈 0.01）, while the protein expression decreased after nine hours （compared with the normal group, P 〈 0.01）. Fetuin-A expressions were negatively correlated with the liver pathological scores and TNF α levels. Conclusion In our mouse FHF model, fetuin-A is a possible protective factor for liver damage.