粤糖系列甘蔗品种遗传多样性的SSR分析

SSR Analysis of Genetic Diversity in YT Series Sugarcane Varieties

从56对SSR引物中筛选出18对多态性较好的引物,对18个粤糖系列甘蔗品种的遗传多样性进行分析,结果表明,每对引物的多态性位点平均为13.8个,多态性位点率为64.6%。18个甘蔗品种的遗传相似系数在0.5555～0.7597之间,平均为0.6762,遗传多样性属中等水平。遗传相似系数分析认为,粤糖83-271与粤糖93-159亲缘关系最近(GS=0.7597),其次是粤糖81-3254与粤糖92-1287(GS=0.7467)。聚类分析在D=0.6300水平上把18个粤糖品种分为2大类,在系谱中亲缘关系密切的品种大多数都可以聚在一起。

Genetic diversity in 18 prominent YT series sugarcane varieties was studied using 18 pairs of polymorphism primers selected from 56 pairs of SSR primers. The results showed that the average polymorphic loci per pair of SSR primers was 13.8, and percentage of polymorphism loci was 64.6%. The value of Dices genetic similarity index of 18 varieties varied from 0.5555 to 0.7597, with an average of 0.6762, which implies that the genetic diversity among the YT varieties analyzed is at middle level. Genetic similarity index indicated genetic relationship of YT83-271 and YT93-159 was the closest, and followed by that of YT81-3254 and YT92- 1287. The 18 varieties were clustered into two groups with a threshold of D=0.6300. Most of the varieties with close pedigree relationship could be clustered into the same group.