奥尼罗非鱼5种组织中4种同工酶的研究

Studies on four isozymes from five tissues of hybrid Tilapia (Tilapia nilotica♀×T.aurea♂)

用聚丙烯酰胺凝胶垂直梯度凝胶电泳法对奥尼罗非鱼肾脏、肝脏、眼睛、肌肉和心脏等5种组织的酯酶(EST)、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)、超氧化物歧化酶(SOD)进行了初步研究,并分析了这4种酶的同工酶位点及酶谱表型。结果表明:奥尼罗非鱼的4种同工酶系统具有明显的组织特异性;EST由7个基因位点编码;LDH酶带多于大多数硬骨鱼典型的5条酶带;MDH和SOD都具有线粒体型和上清液型2种类型。

By means of the vertical polyacrylamide gel gradient electrophoresis, four isozymes （EST, LDH, MDH and SOD） from five tissues （ kidney, liver, eye, muscle and heart） of hybrid Tilapia were studied. And their locis and phenotypes were also analyzed. The results showed that four isozymes presented obvious tissue specificity. EST isozymes coded by seven locis＇s genes; the protein bands of LDH isozymes were more than 5 which LDH phenotypes of most of the typical teleosts have ; the phenotypes of both SOD and MDH had two types, mitochondria type and supernatant type, in hybrid Tilapia.