摘要
使用半定量 RT-PCR 方法检测了鲮各组织中 GH mRNA 的表达,并分析重组鲮 IGF-Ⅰ处理后鲮 GH 表达变化情况。结果表明:鲮的 GH mRNA 表达具有明显的组织特异性,在鲮的肝、肾、脾、肌肉、肠、鳃、性腺、心脏和皮肤中都未检测到 GH mRNA 表达,只在鲮脑中检测到 GH mRNA 表达;经重组鲮 IGF-Ⅰ处理后,鲮脑中GH mRNA 稍有升高,但在肝和肌肉中仍未检测到 GH mRNA 表达;重组鲮 IGF-Ⅰ处理前后,鲮血清中 GH 浓度变化不明显。
The study was conducted to examine the expression of GH mRNA in different tissues of mud carp by semi-quantitative RTPCR and the effects of recombinant mud carp insulin-like growth factor- I （ rmcIGF- I ） on expression of GH in mud carp. GH mRNA detection result of brain of mud carp was positive, and the other tissues detections results were negative, including liver, kidney, spleen, muscle, intestine, gill, gonad, heart and skin. GH mRNA levels increased a little in brain in response to rmcIGF- I , but the treated fish exhibited no obvious change in sera GH concentration following rmcIGF- I injection.
出处
《南方水产》
2006年第5期19-24,共6页
South China Fisheries Science
基金
广东省自然科学基金(033102)
