痹肿消汤对大鼠胶原诱导性关节炎滑膜肿瘤坏死因子-α基因的影响

Effect of Bizhongxiao Decoction on Synovial Tumor Necrosis Factor-α Gene in Rats with Collagen-II Induced Arthritis

目的观察痹肿消汤对大鼠胶原诱导性关节炎肿瘤坏死因子-(αTNF-α)mRNA的影响,从基因水平探讨类风湿关节炎的发病机制及痹肿消汤对胶原诱导性关节炎大鼠的抗炎作用。方法将34只大鼠随机分为3组,皮下注射II型胶原蛋白配合完全弗氏佐剂诱导关节炎模型,采用半定量RT-PCR法测定胶原诱导性关节炎大鼠滑膜细胞TNF-αmRNA的表达。结果TNF-αmRNA在正常组有少量表达;25、45 d模型组TNF-αmRNA表达明显高于正常组(P<0.05),45 d模型组表达高于25 d模型组(P<0.05);给药后,痹肿消汤组的TNF-αmRNA表达明显低于模型组(P<0.05),在痹肿消汤组中,前后比较,45 d TNF-αmRNA的表达低于25 d(P<0.05)。结论在胶原诱导性关节炎大鼠中痹肿消汤能减少TNF-αmRNA的表达,提示痹肿消汤在治疗类风湿性关节炎中有抗炎作用,从而达到治疗作用。

Objective To observe the effect of Bizhongxiao Decoction（BZXD） on synovial tumor necrosis factor-α（TNF-α）mRNA of arthritis（CIA） induced type II collagen in rats and explore the pathogenesis of rheumatoid arthritis and anti-inflammatory effect of BZXD on CIA. Methods The 34 rats were randomly divided into 3 groups to establish the arthritis models by subcutaneous in-jection of type II collagen and complete Freund＇s adjuvant. The expression of synovial TNF-αmRNA were detected by the methods of RT-PCR. Results TNF-α in normal group expressed slightly;expression of TNF-α in model group at 25 days and 45 days was higher than that in normal group（P〈0.05）, expression in model group at 45 day was higher than that in model group at 25 d （P〈0.05） ; After administration, the expression of TNF-αmRNA in BZXD group was lower than that in model group（P〈 0.05）. While compared in the same BZXD group, the expression in 45 days group was lower than that in 25 days（P〈0.05）. Conclusion BZXD can decrease the expression of TNF-αmRNA in CIA, which indicates that the decoction acts on anti-inflammation in the treatment of RA.