不同剂量甘露醇对大鼠缺血性脑水肿的治疗作用

The effectof differentdose of mannitol on treatmentof brain edema

为探讨甘露醇治疗脑水肿的效果及最佳治疗方案 ,采用线栓法阻断大鼠大脑中动脉造成缺血性脑水肿 ,分别于缺血后 12 h、16h、2 0 h、2 4 h给予甘露醇一次 ,和于缺血后 12 h开始用甘露醇 ,每隔 2 h一次 ,用三次、五次和七次 ,甘露醇用量为 0 .5g/ Kg体重 ,对照组为缺血后 12 h、16h、2 0 h、2 4 h未用甘露醇。用脑组织含水量、病理变化及梗塞病灶面积三个指标来观察水肿的程度。结果 :甘露醇对大鼠缺血性脑水肿的治疗作用随着应用次数的增加逐渐减少 ,连用 7次后脑水肿反而增加。提示临床使用甘露醇仅须在急需时短期应用 。

The aim of our study was to investigate the effectof mannitol on treatment of brain edema.Focal ischemic brain edema model was established by left middle cerebral artery occlusion(LMCAO) .The ratswere divided into 1 1 group.Each ratwasintravenously injected with2 0 % mannitol(0 .5 g/ kg within1 0 minutes) .The dose of mannitol was differ- ent.One dose of mannitol at1 2 h,1 6 h,2 0 h,2 4h after L MCAO,three doses,five doses,seven doses of mannitol at 2 4h after L MCAO.Again respective control animals were 1 2 h,1 6 h, 2 0 h,2 4h after L MCAO.Brain water content(BWC) ,morphological changes and ischemic size were used to evaluate the brain edema.The result showed that the BWC reduced in damaged brain atone dose,three doses,five doses of mannitol,(P<0 .0 1 ) and wasincreased at seven doses of mannitol.The change of morphological was in accord with BWC in dam- aged brain.It indicated that:BWC and pathology study are useful in monitoring brain ede- ma.Multiple-dose of mannitol will aggravate brain edema.