HPLC法测定江南卷柏片中穗花杉双黄酮的含量

Determination of Amentoflavone in Jiangnan Spikemoss Tablets with HPLC

目的：建立高效液相色谱法测定江南卷柏片中穗花杉双黄酮的含量。方法：C18柱（250mm×4．6mm，51μm）；流动相：乙腈（A）-0.5％冰醋酸溶液（B），梯度洗脱；检测波长：338nm；流速：1ml/min。结果：穗花杉双黄酮在5.25～262．5μ/ml间，峰面积与其质量呈良好的线性关系，回归方程为A=62621C＋39、79，r^2=1；平均回收率为97．1％，RSD为1．9％。结论：该方法可以用来控制江南卷柏片的质量，方法简便、准确。

Objective：to establish a HPLC method to determine amentoflavone in Jiangnan Spikemoss Tablets, Methods：detective conditions are as follows ： C 18 Column （ 250mm ×4, 6 mm, 5 μm） ; mobile phase ： ace （ A ） - 0. 5 % glacial acetic acid （ B ）, gradient elution ; detective wavelength ： 338nm ; flow rate ：1 ml/min. Results ： area of the peak showed a good linear relationship with content within the range of 5.25 -262.5μg/ml, regression equation： A = 62621C ＋ 39.79, r^2 = 1 with the average recovery rate being 97.1%, RSD = 1. 9%. Conclusion：the method is convenient and accurate for determination of amentoflavone in Jiangnan Spikemoss Tablets.