Amino Acids Production from Fish Proteins Hydrolysis in Subcritical Water

The hydrolysis technology and reaction kinetics for amino acids production from fish proteins in subcritical water reactor without catalysts were investigated in a reactor with volume of 400 ml under the conditions of reaction temperature from 180-320℃, pressure from 5-26 MPa, and time from 5-60 rain. The quality and quantity of amino acids in hydrolysate were determined by bioLiquid chromatography, and 17 kinds of amino acids were obtained. For the important 8 amino acids, the experiments were conducted to examine the effects of reaction temperature, pressure and time on amino acids yield. The optimum conditions for high yield are obtained from the experimental results. It is found that the nitrogen and carbon dioxide atmosphere should be used for leucine, isoleucine and histidine production while the air atmosphere might be used for other amino acids. The reaction time of 30 rain and the experimental temperature of 220℃, 240℃ and 260℃ were adopted for reaction kinetic research. The total yield of amino acids versus reaction time have been examined experimentally. According to these experimental data and under the condition of water excess, the macroscopic reaction kinetic equation of fish proteins hydrolysis was obtained with the hydrolysis reaction order of 1.615 and the rate constants being 0.0017, 0.0045 and 0.0097 at 220℃, 240℃ and 260℃ respectively. The activation energy is 145.1 kJ·mol^- 1.

为从在没有催化剂的 亚critical 水反应堆的鱼蛋白质的氨基酸生产的水解作用技术和反应动力学从 180-320 °C 在反应温度的条件下面与 400 ml 的体积在一个反应堆被调查，从 5-26 MPa 的压力，并且从 5-60 min.The 质量和在 hydrolysate 的氨基酸的数量的时间被简历液相色谱决定，并且17种氨基酸被获得。为重要 8 氨基酸，实验被进行在氨基酸产量上检验反应温度，压力和时间的效果。为高产率的最佳条件从试验性的结果被获得。当时，氮和二氧化碳空气应该被用于白氨酸，异白氨酸和组氨酸生产，这被发现空气空气，可能被用于另外的氨基酸。30 min 的反应周期和 220 ° C， 240 ° C 和 260 ° C 的试验性的温度为反应被采用运动研究。氨基酸对反应周期的全部的收益试验性地被检验了。根据这些试验数据并且在水过量的条件下面，鱼蛋白质水解作用的宏观的反应动力学方程与 1.615 的水解作用反应级数和是的速度常数被获得 0.0017,0.0045 和 0.0097 在 220 ° C， 240 ° C 和 260 ° C 分别地。激活精力是 145.1 kJ · m ol^(-1) 。