蝴蝶兰组织培养及水培移栽技术

Tissue Culture of Phalaenopsis and Transplantation Technique by Water Culture

为探讨水培法移栽蝴蝶兰组培苗的可行性及其最佳营养液配方,以蝴蝶兰叶片为外植体,建立了植株再生系统,并分析5种配方营养液对蝴蝶兰组培苗生长的影响.结果表明,实验中最佳蝴蝶兰类原球茎诱导培养基为[MS+0.4 mg/Lα-萘乙酸(NAA)+6 mg/L 6-苄基氨基嘌呤(6-BA)].自制营养液Ⅳ培养的蝴蝶兰植株生长势最强,其大量元素配方为[Ca(NO3)2.4H2O 354 mg/L+KNO3 177 mg/L+KH2PO4 57.5 mg/L+MgSO4.7H2O 185 mg/L].因此,蝴蝶兰组培苗适宜用水培法进行移栽,自制营养液Ⅳ及栽培管理技术能满足蝴蝶兰组培苗生长发育的需求.

In order to study the feasibility of transplantation technique by water culture and the optimal nutrient formulation of Phalaenopsis tissue culture seedlings, the regeneration system from leaf explants of Phalaenopsis was developed, and the effects of five kinds of nutrient solution on the growth of seedlings were analyzed. The results show that the optimum protocorm-like bodies （PLBs） induction medium is [MS ＋ 0.4 mg/L α-naphthaleneacetic acid （NAA） ＋ 6 mg/L 6- benzylaminopurine （ 6-BA ）]. The growth vigor was the best of Phalaenopsis transplants in nutrient solution IV, in which the macro-elements included [Ca （ NO3 ） 2 · 4H2O 354 mg/L ＋ KNO3 177 mg/L ＋ KH2PO4 57.5 mg/L ＋ MgSO4 · 7H2O 185 mg/L]. Therefore,it is suitable to transplant Phalaenopsis tissue culture seedlings by water culture using nutrient solution IV and the cultivating technique can meet the requirements of growth and development of seedlings.