HBV X基因/C基因启动子变异在重型肝炎病人的发生率

PREVALENCE OF CORE PROMOTER MUTATIONS OVERLAPPING IN X-GENE OF HEPATITIS B VIRUS IN CHINESE PATIENTS WITH FULMINANT HEPATITIS

目的:了解重型乙型肝炎病人乙型肝炎病毒(HBV)X 基因/C 基因启动子(BCP)变异发生情况。方法:用错配聚合酶链反应(PCR)限制性片段长度多态性分析(RFLP),检测中国不同地区58例重型乙型肝炎病人 HBV 毒株 BCP 区变异,并与前 C 区变异情况比较,部分病例经测序证实。结果:58例中可见 HBV 毒株 BCP 区和/或前 C 区变异者43例(74%),BCP 区第2个 AT 丰富区的一对点突变核苷酸(nt)1762碱基由 A→T和1764碱基由 G→A变异者26例(45%),其中19例与前C区变异共存,单一 BCP 区变异者7(12%)例。结论:中国重型乙型肝炎病人感染的 HBV 毒株 BCP 区和前 C 区变异常见,变异与重型肝炎发生和发展的确切关系需进一步深入研究。

Objective:To investigate the prevalence of the core promoter and precore variants of hepa- titis B virus(HBV)in Chinese patients with fulminant hepatitis B and its relation with pathogenesis of fulminant hepatitis B.Methods:By using a mismatched PCR in combination with a restriction fragment length polymorphism assay,detection of the core promoter and precore variants in 58 Chinese fulminant hepatitis B was carried out.Results:The core promoter and precore mutations were detected in 43 of 58 patients with fulminant hepatitis.19 of them infected with a mutant with both in precore and core promoter regions.7 of them had a mutant exclusively with mutation in core promoter region.17 of serum samples only have precore mutant.Conclusion:The core promoter and precore mutants are frequently observed in Chinese patients with fulminant hepatitis B which might be correlated with pathogenesis of fulminant hepatitis.