上海等地区慢性丙型肝炎患者中HCV基因型的分布

DISTRIBUTION OF HCV GENOTYPES DETERMINED BY LINE PROBE ASSAY IN PATIENTS WITH CHRONIC HEPATITIS C IN SHANGHAI AREA

目的:探讨上海等地区丙型肝炎患者中 HCV 基因型的分布。方法:以一种新的 HCV 基因分型方法——线探针试验(INNO-LiPA),对来源于上海等地区的96例慢性丙型肝炎患者进行 HCV 基因分型。结果:显示96例患者中以1b 型为主(占77.6%),其次为2a/2c(占8.62%),并发现1a、4a 和4e 基因亚型。82例感染单一型(1b),其他14例为混合感染,有一例慢性丙型肝炎患者感染5个亚型。对其中39例病人应用干扰素(IFN-a)治疗,37.5%感染1b 亚型的患者在治疗期间 HCV-RNA 由阳性转为阴性,而57.1%感染其他型的患者在治疗期间 HCV-RNA 由阳性转为阴性。结论:分型方法操作简便,可作为预测丙型肝炎患者抗病毒治疗疗效方法之一。

Objective:To determine the distribution of hepatitis C virus(HCV)genotypes in patients with chronic hepatitis C(CHC)mainly from Shanghai area.Method:HCV from 96 patients with CHC were genotyped by a new method named Line Probe Assay(INNO-LiPA).Result:The results showed that subtype lb of HCV was the main type(77.6%),2a/2c was the second,and la,4a and 4e were also found in these patients.Eighty-two patients were infected by only one subtype,14 patients were infected by mixed subtypes and in one of them 5 subtypes were found.Thirty-nine patients were treated with a- interferon(IFN-α),and HCV-RNA became negative during the treatment in 37.5% of patients with subtype lb and in 57.1% of patients with any other subtype.Conclusion:INNO-LiPA can be used as one of the simplest methods to predict the effect of antivirus therapy.