感染乙型肝炎病毒MT_2淋巴细胞表达Fas

EXPRESSION FAS IN MT_2 CELLS INFECTED WITH HEPATITIS B VIRUS

目的:观测 T 淋巴细胞——MT_2细胞对乙型肝炎病毒感染的敏感性,了解病毒感染对淋巴细胞表达 Fas 的影响。方法:体外培养 MT_2细胞,用 HBV DNA 阳性血清直接感染。用酶联免疫法和斑点杂交,PCR 法检测感染后细胞病毒复制与表达状况,用酶联免疫法检测 Fas 抗原表达。结果:MT_2细胞于阳性血清感染后第八天可测到 HBeAg、HBsAg 和HBV DNA,同时出现 Fas 表达信号。结论:MT_2细胞对 HBV 易感,HBV 可上调 MT_2表达 Fas,提示通常在乙型肝炎发病中作为效应细胞的淋巴细胞,亦可能成为凋亡牺牲品。

Objective:To obtain MT_2 cells infected with hepatitis B virus and examine the expression of Fas antigen in those MT_2 cells.Methods:A human T-lymphotropic virus type I infected cell Line MT_2 was cultured ovemight,then 0.5ml serum contained hepatitis B virus DNA(HBV DNA),E antigen and S antigen was added to it together with lml of fresh RPMI 1640 medium and incubated for 20-24 hours at 37℃.The cells were washed three time with RPMI 1640 medium without serum,followed by continued incubation.The medium and the cells were harvested with medium changes at 2- 3 day intervals, and HBV DNA,E and S antigen and Fas antigen were detected by dot blot and polymerase chain reaction, ELISA and immunohistochemistry.Results:HBV DNA and S antigen was detected in the culture medium or/and the cells harvested at 8-12 days post-infected.The strong signals of Fas were observed in MT_2 cells infected with HBV.Conclusion:MT_2,a human T cell line was susceptible to hepatitis B virus infection.Increased expression Fas gene in MT,cells infected with HBV indicated that T-lymphocyte is not only effective ceil but also target ceil in the pathogenesis of viral hepatitis.