军曹鱼稚鱼外周血细胞及其形态学观察

Study on the morphology of peripheral blood cells of juvenile cobia Rachycentron canadum

以出膜后第60天的军曹鱼外周血液为材料,利用光镜显微技术研究了其血细胞的种类和形态。外周血液包括5种类型的血细胞,红细胞、淋巴细胞、单核细胞、嗜中性粒细胞和血栓细胞。血细胞计数测得红细胞密度为(2.97±0.82)×109ind.mL-1,白细胞密度为(1.39±0.94)×106ind.mL-1,淋巴细胞、嗜中性粒细胞、单核细胞和血栓细胞在白细胞中所占的比例分别为(53.00±8.51)%、(17.59±4.28)%、(4.78±1.37)%和(24.63±4.08)%。未见到嗜酸性粒细胞和嗜碱性粒细胞。血细胞计数结果表明,红细胞数量远远大于白细胞,白细胞中淋巴细胞数量最多,单核细胞数量最少。实验还观察到处于分裂状态的红细胞、细胞表面有伪足样胞突的小淋巴细胞和细胞表面有许多微绒毛突起的大淋巴细胞、核呈多种形态的单核细胞和中性粒细胞及不同形态的血栓细胞等。

The peripheral blood was collected from cobia （Rachycentron canadum） on the 60th day after hatching, and blood cells were studied by light microscope. Five types of blood cells were observed as follow： erythrocyte, lymphocyte, monocyte, neutrophil and thrombus cells. The erythrocyte counts and leukocyte counts of cobia was （2. 97 ±0. 82） ×10^9ind·mL^-1 and （ 1.39 ±0. 94）×10^6ind·mL^-1, respectively. The percentages of lymphocytes, neutrophils, monocytes and thrombocytes in total leucocytes, were （ 53.00 ±8.51 ） %, （ 17.59± 4. 28 ） %, （4. 78 ±1.37 ） % and （ 24.63 ±4. 08 ） %, respectively. Neither eosinophil nor basophil was found. The quantity of erythrocytes was much larger than that of leucocytes. Among the leucocytes, the lymphocytes were the most abundant, while that of monocytes was the least. The immatured and dividing erythrocyte were found. The small lymphocytes with pseudopod and large lymphocytes with the villus were observed also. In addition, the monocyte, neutrophil and thrombu cells were recognized by different shapes of nucleolus.