二长棘犁齿鲷线粒体细胞色素b基因序列和分子系统发育分析

The sequence analysis of mitochondrial cytochrome b gene and molecular phylogeny of Parargyrops edita

利用PCR技术克隆了二长棘犁齿鲷(Parargyrops edita Tanaka)线粒体细胞色素b(Cyt b)基因,该基因的全长为1140bp,并将该基因与GenBank中犁齿鲷、金头赤鲷、真赤鲷、四长棘鲷、黄牙鲷、平鲷、黑棘鲷和灰鳍棘鲷的Cytb序列进行了比较,共检测到407个核苷酸变异位点,占总序列的35.7%。同时结果也显示,二长棘犁齿鲷与犁齿鲷亲缘关系最近,序列差异仅为1.5%,与传统分类一致,它们应同属于犁齿鲷属;与金头赤鲷和真赤鲷的遗传差异分别为8.3%和8.4%,也处于种间的分化水平,且以较高的置信度(100%)在分子系统发育树上聚为一支,是否应该将它们归为一个属还有待于深入研究。

The sequence of mitochondrial cytochrome b （ Cyt b） gene was amplified from Parargyrops edita using PCR technique and it had a length of 1 140 bp. Then the sequence was aligned with the Cyt b gene sequences of Evynnis japonica, Pagrus auratus, P. major, Argyrops spinifer, Dentex tumifrons, Rhabdosargus thorpei, Acanthopagrus schlegelii and Acanthopagrus berda in GenBank. The results demonstrated that there were 407 nucleotide sites substituted （35.7%）. P. edita and E.japonica were the most related among the 9 Sparidae fishes. Their genetic divergence was only 1.5%, so they two might belong to the same genus （Evynnis）, which was in consistent with that of the traditional morphological taxon. The genetic divergence of P. edita with P. auratus and P. major were 8.3% and 8.4%, respectively, which showed differentiation at the level between strains. Moreover, they assembled one branch in the molecular phylogeny with the most bootstrap value. Whether the Evynnis genus and Pagrus genus belong to the same genus still needs further study.