摘要
通过体外制备血细胞单层的方法,研究了中华绒螯蟹(Eriocheir sinensis)血细胞对蜡状芽孢杆菌(Bacil-luscereus)及鼠红细胞的吞噬能力,并探讨了血清孵育对血细胞吞噬能力的影响。结果表明,以vanHARREV-ALD氏液配制的浓度为100.0mg·mL-1的L-cysteine溶液可以作为中华绒螯蟹血淋巴的抗凝剂,中华绒螯蟹血细胞在20℃下培养1h的存活率可维持在(92.5±0.9)%。中华绒螯蟹血细胞贴壁后呈扁平状,胞质可沿玻片表面迅速伸展并伸出类似于伪足的原生质突起,具有吞噬能力,对蜡状芽胞杆菌吞噬率为(5.1±1.4)%,不能吞噬鼠红细胞,血清孵育不能增强中华绒螯蟹血细胞的吞噬能力。
Phagocytosis, the cellular defensing reactions of Eriocheir sinensis, was studied in vitro using Bacillus cereus and formalinized mouse erythrocytes as test particles. The results showed that 100.0 mg·mL^-1 L-cysteine van HARREVALD＇s solution could anticoagulate hemolymph of E. sinensis. And phagocytosis was studied using a monolayer culture system which maintained cell viability at （92. 5 ± 0. 9）% for 1 hour at 20℃. Many of spread cells were phagocytic cells, which had pseudopodia and could phagocyte B. cereus in vitro. Uptake of the Gram-positive bacteria, B. cereus, was found to be （5.1 ±1.4）%. The formalinized mouse erythrocyte was not phagocytosed at all. Serum incubation did not increase the percentage of uptake of any of the test particles with the haemocytes.
出处
《南方水产》
2007年第6期47-51,共5页
South China Fisheries Science
基金
上海市重点学科建设项目(Y1101)
关键词
中华绒螯蟹
血细胞
吞噬
Eriocheir sinensis
haemocytes
phagocytosis
