胚胎肢芽提取物对体外培养的背根神经节感觉神经元的作用

Effects of embryonic limb buds extract (ELBE) on cultured sensory neurons of dorsal root ganglia from embryonic rats

从大鼠胚胎肢芽中提取生物活性物质,研究其对体外培养的胚鼠背根神经节感觉神经元的作用。方法:剖腹取孕10～15天的SD大鼠胚胎,将胚胎肢芽的提取物加至胚鼠背根神经节感觉神经元的体外培养基中,同单纯培养基培养作对照。接种后,采用相差倒置显微镜、电镜、台盼蓝染色计数和乙酰胆碱脂酶(AchE)定量测定等技术观察培养细胞的生长、功能、超微结构和成活率的变化。结果:实验组细胞的成活率和轴突的长度明显优于对照组(P<0.01);AchE的含量明显多于对照组(P<0.01)。电镜下实验组线粒体、核糖体和高尔基复合体等细胞器的超微结构和功能状态也明显优于对照组。结论:胚胎肢芽提取物(ELBE)具有较强的维持神经元存活,促进轴突生长的神经营养因子(NTFs)活性。

Objective:To study the neurotrophic activity of SD rat embryonic limb buds extract (ELBE) towards cultured sensory neurons of dorsal root ganglia from embryonic rats. Methods: Rat embryoes of 10~15 days were taken from SD rats. The bioactive elements from their limb buds were extracted by biochemical methods. ELBE was then added into the cultures of sensory neurons in dorsal root ganglia from embryonic rats of 2 weeks. Sensory neurons cultured in single culture medium without ELBE served as control. Phase-contrast microscope and electron microscope observations, cell dyeing and true acetylcholinesterase (AchE) measurement were used to evaluate the survival, activity and ultrastructure of the cultured neurons. Results: The neurons cultured with ELBE had a higher survival rate, longer axons and higher AchE content than the control ones (P<00. 01). The ultrastructure of mitochondria, ri-bosomes and Gorgi's complex was better. Conclusion: Embryonic limb buds extract (ELBE) has strong neurotrophic activities in maintaining survival of neurons and promoting growth of axons.