一种新的肺纤维化造模方法

A NEW METHOD OF INDUCED PULMONARY FIBROSIS

目的：探寻一种更为简便易行的肺纤维化造模方法，以普及对本病的研究，提高其防治水平。方法：将４０只Ｗｉｓｔａｒ大鼠随机分为２组，造模组３０只大鼠平均分为Ａ、Ｂ、Ｃ３个亚组，依次于雾化吸入平阳霉素后ｄ７、１４、２８处死，测肺系数、体重，制病理切片光镜观察，并与正常对照组比较。结果：４周实验完成后，光镜下观察肺组织呈纤维化改变；造模Ｃ组体重为２２６±１７７８ｇ，比对照组（２６８±２２８０ｇ）生长缓慢，存在极显著差异（Ｐ＜００１）；造模组的肺系数为３５９±０８７，比对照组（１０７±０６８）增高，存在极显著差异（Ｐ＜００１），此改变均与肺纤维化相符。结论：雾化吸入平阳霉素是一种简便易行的肺纤维化造模方法，适于各层次医务人员掌握应用。

Objective:To establish an animal model for pulmonary fibrosis research and popularize the prevention investigation of this disease.Methods:Wistar rats were divided into normal( n =10) and test( n =30) groups.Domestic made bleomycin were aerosolly inhalated to the operated groups and the rats were separately killed after 7,14 and 28 days.After the experiments,all the rats were weighted and its lung slices were taken and observed by optical microscope.The data and photographs were analyzed and compared between test and normal groups.Results:After four weeks,the lung tissue of the test groups showed induced fibrosis.Conclusion:Bleomycin induced pulmonary fibrosis in rats by aerosol inhalation was availible and it was easier to establish than before.This method meets the requirement of reserchers who are not speciality in the field.Thus,it will be much benefit to increase the level of the study for pulmonary fibrosis.