肝细胞刺激物质对感染鸭乙型肝炎病毒的雏鸭体内抗病毒作用的实验研究

Anti-virus effect of hepatocyte stimulating substance (HSS) on duck hepatitis B virus in vivo

以感染鸭乙型肝炎病毒（ＤＨＢＶ）的北京雏鸭为体内实验动物模型，观察了肝细胞刺激物质（ＨＳＳ）对鸭乙型肝炎病毒的抗病毒作用。１日龄北京鸭实验感染ＤＨＢＶ，７天后血清ＤＨＢＶＤＮＡ阳性，给予药物治疗１０天，用斑点杂交方法检测用药前后血清ＤＨＢＶＤＮＡ，分析药物对血清ＤＨＢＶＤＮＡ是否有抑制作用，结果显示：ＨＳＳ大、小两个剂量组（分别为５０ｍｇ／ｋｇ／ｄ和２００ｍｇ／ｋｇ／ｄ）均对ＤＨＢＶＤＮＡ有抑制作用，大剂量组出现ＤＨＢＶＤＮＡ抑制作用时间早于小剂量组，用药５天、１０天及停药３天血清ＤＨＢＶＤＮＡ中位抑制率显著高于生理盐水对照组（Ｐ＜０．０５）；两个剂量组均无停药反跳；生理盐水组用药前后ＤＨＢＶＤＮＡ的Ａ值比较差异无显著性意义（Ｐ＞０．０５）。停药后３天肝组织病理光镜及电镜均显示ＨＳＳ组病变较生理盐水组轻。

We used one day old Beijing ducklings infected by duck hepatitis B virus as experimental model to observe antivirus effect of HSS. Beijing ducklings hatched within one day were injected intravenously with duck hepatitis B virus (DHBV). Positive DHBV-DNA in ducklings' sera were detected 7 days later. Serum samples were obtained from all ducklings before, during and after drug treatment at different times and stored at -70℃. The collected samples were detected DHBV-DNA by dotblot hybridization test at the same time. The therapeutic effects were evaluated by OD value of dotblot and by the inhibitory rate of DHBV-DNA. The results showed that HSS (with 50mg/kg/day and 200mg/kg/day) were effective in inhibition of DHBV-DNA during and after drug treatment, especially in the HSS group of large dosage. The inhibitory effect appeared early and high dynamic. The inhibitory rate of DHBV-DNA during and after drug treatment were higher than the NS group (P<0.05). No inhibitory effect was observed in N S group. The hepatic biopsy showed HSS groups had milder demage than NS group, indicaing that the HSS could inhibit the replication of DHBV-DNA in Beijing ducklings.