输血后丙型肝炎病毒非结构区抗体和丙氨酸转氨酶的动态分析

Dynamic study of antiNS5 and ALT in posttransfusion hepatitis C

利用重组的丙型肝炎病毒非结构区（ＨＣＶＮＳ５）抗原建立了酶免疫试验（ＥＩＡ），对２５例输血后丙型肝炎进行了不同区抗体及丙氨酸转氨酶（ＡＬＴ）的动态研究，同时对１５６例慢性丙型肝炎患者血清进行ＨＣＶＲＮＡ和抗－ＮＳ５平行检测，两者符合率为６４．１％。抗－ＮＳ５抗体首次检出时间为３０～５７５天（１８２．９±１６８．５），晚于ＡＬＴ异常和其他区抗体的出现时间。在感染后１，３，６，１２和２４个月后抗－ＮＳ５的阳性率分别为２８％，４０％，５２％，６８％和７６％。抗－ＮＳ５的动态变化类型为四种：一过性阳性、间歇性阳性。

In order to study the character and dynamic changes of HCV NS5 antigen in posttransfusion hepatitis C, EIA was established with fusion protein, and different groups of population were investigated. The results showed the positive rate of whole blood donor, normal people, chronic hepatitis C patients and post transfusion hepatitis C patients is 0.0%, 1.66%, 50.7% and 70.5%, respectively. Serial blood samples of 25 cases of acute or chronic posttransfusion hepatitis C were detected, and the results indicated that the antibody of NS5 appeared relatively late, and the serum conversion time was 182.9±168.5 days. The antiNS5 positive rate in 1, 3, 6, 12 and 24 months after HCV infection was 28%, 40%, 52%, 68% and 76%, respectively. The positive rate of antiNS5 in sera with HCV RNA was 61.9%. Analysis on the appearance of antibody to the different antilody region of the gene, ALT and HCV RNA showed the dynamic changes of antiNS5 were coresponding to the serum ALT in some cases, indicating antibody of NS5 appear later and may reflect the disease activity to some extent. The dynamic changes of antiNS5 are of four kinds: transient positive, intermittent positive, persistent positive and persistent negative in during the two years period.