合成人IgE肽抗血清抑制Ⅰ型变态反应的实验研究

Experimental Study on the Inhibitory Effects of Antisera Induced by Human IgE Synthetic Peptides on Type I Hypersensitivity

作者设计合成了5个人IgE多肽片段,分别交联载体蛋白后免疫小鼠,诱生的抗血清有2个在ELISA中对人与鼠IgE分子有交叉反应,3个无反应。5个抗血清均显示有中等程度的被动皮肤过敏反应（PCA）抑制作用。被测试的2个抗血清在大鼠肥大细胞被动致敏试验中也显示了抑制作用。研究结果初步表明,采用人IgE分子受体结合部位的适当残基序列的合成肽疫苗,免疫动物所诱导的抗体可抑制Ⅰ型变态反应。

Five peptides derived from human IgE C e3 and C s4 domain have been synthesized and conjugated by glutaradehyde to protein carriers (BSA or KLH). Antisera to these conjugates have been prepared and assayed for their biological activity and the inhibitory effects on type I hypersensitivity. Using ELISA, the antisera induced by each conjugate could react strongly with their self-peptide. Anti N-B, anti S-B could react with both human and mouse IgE. The other three antisera showed no activity to either IgE molecule. In rat passive cutaneous anaphylaxis (PCA), five antisera had PCA suppressing activity of different degrees (1 : 4, 46%-66%). Anti N-B and anti S-B can also suppress rat mast cell passive sensitization (1 : 4, 62.5%, 61-7%). The preliminary results indicate that the antisera, which were induced by IgE peptide vaccines derived from proper sequences of receptor binding sites on IgE molecule, can suppress hypersensitivity, showing the potentiality of IgE peptide vaccine as a general treatment preparation for Type I hypersensitivity.