反相毛细管电色谱中两种电渗流测定方法的比较

Comparison of Electroosmotic Flow Measuring by Two Methods in Reversed Capillary Electrochromatography

在毛细管电色谱中电渗流的测定方法有许多种,常用的是示踪剂法。本文将硫脲示踪剂法和迭代分析法测定的电渗流结果加以对比分析,结果表明:在不同有机调节剂体积分数、不同电压、不同电解质浓度下,虽然用两种方法测得的电渗流随诸因素的变化规律符合电渗流变化的一般规律,但强极性硫脲分子存在电迁移现象,使测得的电渗流较真实值有所偏离。偏离的大小随有机调节剂体积分数、电解质浓度等实验条件变化。

There are many methods in measuring electroosmotic flow. The common one is to use a marker. In this article, the dead time was obtained by two methods. One was by using thiourea as a marker, from linear and the other was regression of benzene homologue. Based on the dead time obtained from these two methods, characteristics of electroosmatic flow in reversed phase capillary electrochromatogrphy were investigated under the conditions of different organic modifier, separating voltage and ionic strength. The results showed that they obeyed the common law, but the thiourea marker had the characteristics of migration and the electroosmatic flow measuring by this method has some weak change due to the different conditions.