溴甲酚紫、溴甲酚绿光度法测定阿昔洛韦

Spectrophotometric Method for Determination of Aciclovir with Bromocresol Purple and Bromocresol Green

在pH3．30的NH2CH2COOH-NaCl-HCl缓冲介质中，阿昔洛韦（Aciclovir，ACV）可与溴甲酚紫（BCP）、溴甲酚绿（BCG）反应，形成离子缔合物，溶液颜色发生明显变化，其最大褪色波长分别为428nm（BCP-ACV）、450nm（BCG-ACV），在此波长处，阿昔洛韦的浓度与溶液褪色程度呈良好线性关系，可用分光光度法测定。在BCP-ACV、BCG-ACV体系的最大褪色波长处，ACV的浓度分别在0～2．69×10^-5 mol／L、0～2．58×10^-5 mol／L范围内遵守比耳定律，表观摩尔吸光系数分别为2．53×10^4 L·mol^-1·cm^-1、1．79×10^4 L·mol^-1·cm^-1，检出限分别为5．10×10^-7 mol／L、7．13×10^-7 mol／L。方法用于药品、血浆及尿液中阿昔洛韦的测定，结果满意。

A sensitive spectrophotometric method is developed for determination of aciclovir. In pH 3.30 NH2CH2COOH-NaCl-HCl buffer medium, aciclovir （ACV） reacts with bromocresol purple （BCP） or bromocresol green （BCG） to form an ion-association complex, the colours of solutions change obviously, the maximum fading wavelengths are at 428 nm（BCP-ACV） and 450 nm（BCG-ACV）, respectively, and the fading intensity is proportional to the concentration of aciclovir. For BCP-ACV and BCG-ACV systems, the Beer＇s law are obeyed in the concentration range of 0-2.69×10^-5 mol/L and 0-2.58×10^-5 mol/L, the apparent molar absorptivity are 2.53×10^4 L·mol^-1·cm^-1 and 1.79×10^4 L·mol^-1·cm^-1 , and the detection limits of ACV are 5.10×10^-7 mol/L and 7. 13×10^-7 mol/L, respectively. The method has high sensitivity and fairly good selectivity, and has been applied to the determination of aciclovir in medicine samples and human serum and human urine with satisfactory results.