肿瘤坏死因子α和干扰素α对膀胱癌患者LAK细胞增殖和细胞毒作用的影响

Effect of TNFα and IFNα on the proliferation and cytotoxicity of lymphokineactivated killer cells in patients with bladder cancer

为探讨联合应用白细胞介素２（ＩＬ２）和干扰素（ＩＦＮ）或肿瘤坏死因子（ＴＮＦ）对膀胱癌患者ＬＡＫ细胞的增殖和细胞毒作用的影响，采用淋巴细胞分离液梯度离心法从２１例膀胱癌患者外周血分离ＬＡＫ细胞并用ＩＬ２激活培养，细胞于９６孔细胞培养板用细胞计数方法观察不同浓度ＴＮＦα和ＩＦＮα对细胞增殖的影响，用膀胱癌细胞系ＢＩＵ８７和ＥＪ细胞作为靶细胞，用ＭＴＴ法测定ＬＡＫ细胞的细胞毒。结果：ＴＮＦα呈剂量依赖性地加强由ＩＬ２诱导的ＬＡＫ细胞增殖，１０００Ｕ／ｍｌ的ＩＦＮα于４８小时也对ＬＡＫ细胞产生刺激作用，用５０００Ｕ／ｍｌ的ＴＮＦα可增强ＬＡＫ细胞对ＥＪ和ＢＩＵ８７细胞的细胞毒杀伤，ＩＦＮα对此则无明显影响。结果提示ＴＮＦα和ＩＦＮα增强膀胱癌患者ＬＡＫ细胞的增殖并在细胞毒杀伤中起重要作用，对临床膀胱癌的免疫治疗提供一定依据。

LAK cells were generated by ficoll paque density centrifugation from the patients with bladder cancer and cultured in the medium containing IL2. LAK cell proliferation was assayed in the pressence of various concentrations of either TNFα or IFNα by cell count in 96 wells plates.Bladder cancer cell lines BIU87 and EJ were cultured as target cells and cytotoxicity of LAK cells was determined by MTT assay.The proliferation of LAK cells induced by IL2 was enhanced by TNFα in a dose responsive fashion.The direct growth support for the LAK cells was also observed at IFNα concentration of 1 000 U/ml for 48h.TNFα (5 000U/ml) resulted in an increase in cytotoxicity activity against BIU87 and EJ cells.However the LAK cell cytotoxicity treated with IFNα was not statistically signigicant.These results suggest that TNFα and IFNα enhance the proliferation,activation and the antitumour cytotoxicity of LAK cells in patinets with bladder cancer.