摘要
为探讨联合应用白细胞介素2(IL2)和干扰素(IFN)或肿瘤坏死因子(TNF)对膀胱癌患者LAK细胞的增殖和细胞毒作用的影响,采用淋巴细胞分离液梯度离心法从21例膀胱癌患者外周血分离LAK细胞并用IL2激活培养,细胞于96孔细胞培养板用细胞计数方法观察不同浓度TNFα和IFNα对细胞增殖的影响,用膀胱癌细胞系BIU87和EJ细胞作为靶细胞,用MTT法测定LAK细胞的细胞毒。结果:TNFα呈剂量依赖性地加强由IL2诱导的LAK细胞增殖,1000U/ml的IFNα于48小时也对LAK细胞产生刺激作用,用5000U/ml的TNFα可增强LAK细胞对EJ和BIU87细胞的细胞毒杀伤,IFNα对此则无明显影响。结果提示TNFα和IFNα增强膀胱癌患者LAK细胞的增殖并在细胞毒杀伤中起重要作用,对临床膀胱癌的免疫治疗提供一定依据。
LAK cells were generated by ficoll paque density centrifugation from the patients with bladder cancer and cultured in the medium containing IL2. LAK cell proliferation was assayed in the pressence of various concentrations of either TNFα or IFNα by cell count in 96 wells plates.Bladder cancer cell lines BIU87 and EJ were cultured as target cells and cytotoxicity of LAK cells was determined by MTT assay.The proliferation of LAK cells induced by IL2 was enhanced by TNFα in a dose responsive fashion.The direct growth support for the LAK cells was also observed at IFNα concentration of 1 000 U/ml for 48h.TNFα (5 000U/ml) resulted in an increase in cytotoxicity activity against BIU87 and EJ cells.However the LAK cell cytotoxicity treated with IFNα was not statistically signigicant.These results suggest that TNFα and IFNα enhance the proliferation,activation and the antitumour cytotoxicity of LAK cells in patinets with bladder cancer.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
1997年第12期710-712,共3页
Chinese Journal of Urology
基金
甘肃省自然科学基金