培养基影响大肠杆菌产L-天冬酰胺酶的研究

Effect of Culture Medium on L-asparaginase Porduction by E.coli

研究培养基的成分及添加量对大肠杆菌产生抗癌药物—L-天冬酰胺酶的影响。通过对大肠杆菌培养基中碳源、氮源以及微量元素的讨论,得知该菌株以蔗糖为碳源,添加量为0.7%,以0.5%牛肉膏+1.0%蛋白胨为氮源,添加0.01%的硫酸亚铁为微量元素,所产L-天冬酰胺酶的比酶活达到352.54U/g,相比基本培养基培养提高了31%。

The effects of the components of culture medium on the production of L-asparaginase, an anti-cancer drug fermented by E.coli, were studied. Results showed that the highest specific activity of L-asparaginase （352,54U/g） was achieved under such conditions： 0.7% of sucrose as carbon source, 0,5% of beef extract and 1,0% of peptone as nitrogen source and 0,01% of FeSO4 as trace element. The yield of L-asparaginase was 31% higher than that by basic medium fermentation.