TSPmRNA表达、血浆PF4水平与甲状腺乳头状癌颈部淋巴结转移的关系

Relationship between expression of TSPmRNA,concentration of Plasma PF4 and cervical lymph node metastasis in papillary thyroid carcinoma

目的探讨血小板反应素(TSP)mRNA表达、血浆血小板第四因子(PF4)水平与甲状腺乳头状癌(PTC)颈部淋巴结转移的关系。方法应用逆转录多聚酶链反应(RT-PCR)技术检测40例PTC癌组织和10例癌旁正常组织TSP1和TSP2mRNA的表达,并用酶标记免疫分析法(ELISA)测定40例PTC患者、20例健康体检者(对照组)的血浆PF4水平。结果(1)PTC癌组织TSP1mRNA、TSP2mRNA阳性表达率分别为45%、47.5%,它们均明显低于正常组织(80%)(P<0.01),而且TSP1mRNA、TSP2mRNA阳性表达PTC组的颈部淋巴结转移率均明显低于TSP1mRNA、TSP2mRNA阴性表达PTC组(33.3%vs 90.9%、42.1%vs 85.7%,P<0.01)。(2)正常对照组、无淋巴结转移的PTC组和伴淋巴结转移的PTC组间的PF4血浆浓度呈线性递减趋势,其中无淋巴结转移组的PF4血浆浓度(2.96±0.21 ng/ml)高于转移组(0.98±0.17 ng/ml)(P<0.05)。(3)TSP1mRNA、TSP2mRNA阳性表达PTC组的血浆PF4水平分别明显高于TSP1mRNA、TSP2mRNA阴性表达组(2.36±0.91 ng/ml vs 1.11±0.60 ng/ml,2.15±1.00 ng/ml vs 1.23±0.72 ng/ml)(P<0.01)。结论缺乏TSP1mRNA、TSP2mRNA表达及血浆低水平的PF4诸因素可能为促进PTC发生颈部淋巴结转移起到了推波助澜的作用,TSP1、TSP2与PF4在抑制PTC颈部淋巴结转移中起着互相协同的作用。

Objective To explore the relationship between expression of thrombospondin（TSP） mRNA, concentration of Plasma PF4 and cervical lymph node metastasis in papillary thyroid carcinoma（PTC） .Methods RT-PCR technique was utilized to detect the expression of TSP1 and TSP2 mRNA in 40 cases of PTC and 10 cases of nomal tissue surrounding carcinoma. Meanwhile enzymelinked immunosorbant assay（ELISA） technique was used to measure the concentration of human platelet factor 4（PF4） in plasma of every 40 patients with PTC and 20 healthy persons taken as control. Results （ 1 ） The positive expression rate of TSP1 mRNA of PTC was 45% .The positive expression rate of TSP2mRNA was 47.5% .The positive expression rate of normal tissue was 80% .Either the positive expression rate of TSP1 or TSP2 mRNA of PTC tissues was significandy lower than that of nomal tissues（ P 〈 0.01）. Furthermore the metastatic rate of cervical lymph node of PTC with TSPlmRNA or TSP2mRNA positive expression was signffcantly lower than that of PTC with TSP1mRNA or TSP2mRNA negative expression accordingly （33.3 % vs 90.9 %, 42.1% vs 85.7 % ） （ P 〈 0.01 ）. （2） The concentration of PF4 in plasma of normal healthy person,PTC with cervical lymph node metastasis and PTC without cervical lympli node metastasis was in linear progressive decreasing tendency. The plasma concentration of PF4 of PTC without cervical lympli node metastasis（2.96± 0.21 ng/ml） was higher than that of PTC with cervical lymph node metastasis（0.98 ± 0.17 ng/ml） （ P 〈 0.05）. （3） The plasma concentration of PF4 of PTC with TSPlmRNA or TSP2mRNA positive expression was higher than that of PTC with TSP1mRNA or TSP2mRNA negative expression（2.36 ± 0.91ng/ml vs 1.11 ± 0.60 ng/ml,2.15 ± 1.00 ng/ml vs 1.23 ± 0.72 ng/ml） （ P 〈 0.01 ）. Conclusion Deficiency of TSPlmRNA or TSP2mRNA expression and low plasma level of PF4 may become such promoters as fuel added to the flames in the process of cervical lymph node metastasis of PTC. TSP1, TSP2 and PF4 serve as coordinate factors in the inhibition of cervical lympli node metastasis of PTC.