中国部分地区高致病性猪繁殖与呼吸综合征病毒的分子流行病学研究

Molecular Epidemiology Study on High Pathogenic Porcine Reproductive and Respiratory Syndrome Virus in Some Regions of China

【目的】对来自2006年猪病流行爆发地的组织样品进行多种病毒的检测,确定引起本次流行的致病病原及PRRSV的分子流行病学特征。【方法】利用(RT-)PCR对采集的样品进行PRRSV北美型和欧洲型、猪圆环病毒2型(PCV2)、猪血凝性脑脊髓炎病毒(PHEV)、日本乙型脑炎病毒(JEV)以及瘟病毒的检测,对PRRSV阳性的进行ORF5的序列测定并分析。【结果】检测到16份PRRSV北美型阳性样品,PCV2阳性样品2份,其它病毒均未检到。PRRSV ORF5序列分析表明笔者分离到的PRRSV株可分为2个群。【结论】(1)PRRSV与2006年肆虐中国养猪业的猪病流行有直接关系;(2)2006年PRRSV流行株主要是以JX0612株为代表,但不同遗传特征的PRRSV也在流行。

【Objective】Various viruses were detected from tissue samples of porcine disease epidemic in 2006. Etiology of this epidemic and molecule epidemiology of porcine reproductive and respiratory syndrome virus were determined. 【Method】PRRSV North America type and Europe type,porcine circovirusⅡ,porcine haemagglutinating encephalomyelitis virus,Japanese encephalitis virus and Pestivirus were identified by （RT-）PCR from tissue samples. Sequence of PRRSV ORF5 was amplified and analyzed. 【Result】16 PRRSV North America type positive samples and PCV2 positive samples were detected; other viruses were not detected. Sequence analysis of PRRSV ORF5 indicated that the isolated PRRSV strains could be divided into two groups. 【Conclusion】（1） PRRSV was the causative agent of porcine disease epidemic in 2006. （2） PRRSV prevalence strain was strain represented by JX0612,however defferent genetic characteristic PRRSV strains also spread in China.