斯卑尔脱小麦α-醇溶蛋白基因克隆与序列分析

Cloning and Sequence Analysis of α-Gliadin Genes from Spelt Wheat

【目的】进一步了解斯卑尔脱小麦(Triticum spelta L.)α-醇溶蛋白基因序列信息。【方法】根据已知的普通小麦α-醇溶蛋白基因序列设计引物,采用PCR方法,克隆基因并进行序列分析。【结果】从NGB5149中克隆得到两个α-醇溶蛋白基因序列Gli-Spelt-1和Gli-Spelt-2(GenBank登录号分别为DQ234066和DQ234067)。它们具有α-醇溶蛋白基因的典型结构特征,但Gli-Spelt-1是一个假基因。Spelt-Gli-2编码区全长849bp,编码263个氨基酸。【结轮】氨基酸序列比较显示,Gli-Spelt-1和Gli-Spelt-2与已报道的α-醇溶蛋白序列有较高的一致性。

【Objective】The objective of this study is to get more information on the α-gliadin genes of spelt wheat （Triticum spelta L.）. 【Method】Specific PCR primers were designed based on the known wheat α-gliadin gene sequences,and were used to amplify the coding genes of α-gliadin from spelt wheat. 【Result】The coding regions of two gliadin genes,Gli-Spelt-1 and Gli-Spelt-2,were isolated from spelt wheat NGB5149. Gli-Spelt-1 was a pseudogene due to the stop codons in its coding region. The full coding region of Spelt-Gli-2 was 849 bp,and could be translated into a protein of 263 amino acids. 【Conclusion】 The two cloned gliadin genes have the typical structure of α-gliadin genes. The deduced amino acid sequences comparison suggested that Spelt-Gli-2 has a high degree of identity with the known a-gliadin genes.