桑树组织培养物中1-脱氧野尻霉素的产生规律初探

Research on Production Regularity of 1-deoxynojirimycin in Mulberry Tissue Cultures

为探讨桑树合成1-脱氧野尻霉素(1-DNJ)的代谢途径,采用反相高效液相色谱(RP-HPLC)分析法,研究了桑树品种大10的组织培养物中1-脱氧野尻霉素的产生规律。结果表明:桑树几种组织培养物中1-DNJ的质量分数在无菌苗叶片中为0.309604%,无菌苗中为0.158516%,再生芽中为0.318756%,再生苗中为0.168672%;桑树绿色愈伤组织、疏松愈伤组织、悬浮培养细胞、悬浮细胞诱导愈伤组织中不含1-DNJ。推测1-DNJ产生于由愈伤组织分化为再生芽的阶段。

To investigate the metabolic pathway of 1-deoxynojirimycin （1-DNJ） in the mulberry, the production regularity of 1-DNJ in the tissue cultures of mulberry Da 10 variety was investigated using reversed phase high performance liquid chromatography method. The result showed that the content of the 1-DNJ was 0. 309 604% in aseptic plant leaves, 0. 158 516% in aseptic seedling, 0. 318 756% in regeneration buds, and 0. 168 672% in regeneration plant, respectively. There was no 1-DNJ in green callus, loose callus, suspension cells and induced callus from suspension cells, suggested that the 1-DNJ was produced in the phase of callus differentiation to regeneration buds.