芦荟凝胶对人皮肤成纤维细胞增殖及胶原合成的影响

Effect of aloe gel on cell proliferation and synthesis of collage in cultured human skin fibroblast

目的研究芦荟凝胶(aloe gel,AG)对人皮肤成纤维细胞(human skin fibroblast,HSFb)的增殖及胶原蛋白合成的影响。方法原代培养人皮肤成纤维细胞,传代培养后加入不同浓度的芦荟凝胶作用后,应用流式细胞仪检测细胞周期变化,应用碱水解法检测培养液中羟脯氨酸的含量变化,应用免疫组化法检测芦荟凝胶对成纤维细胞Ⅰ型前胶原合成的影响。结果细胞周期时项分析结果显示5%、10%浓度芦荟凝胶处理组细胞周期S+G2/M期细胞百分率及增殖指数PI值较对照组增高(P<0.05),但在不同时间点检测的细胞周期差异无统计学意义(P>0.05)。各浓度芦荟组在不同时间段时其成纤维细胞培养液中羟脯氨酸含量均明显高于空白对照组(P<0.05)。经10%浓度芦荟凝胶处理后的成纤维细胞Ⅰ型前胶原表达增加(P<0.05)。结论芦荟凝胶能有效促进人皮成纤维细胞的增殖和胶原的合成。

Objective To investigate the effect of aloe gel on cell proliferation and synthesis of collage in cultured human skin fibroblast. Methods The primary human skin fibroblasts were isolated and cultured in vitro,which were treated with aloe gel in different concentration in exponential phase of cell growth. The hydroxyproline in the cultural medium was determined by alkaline hydrolysis. Cell cycle was measured by flow cytometry. The expression of procollagen type Ⅰ was detected by immunohistoehemistry. Results Employing the flow cytometry technique we observed the cell cycle of S＋G2/M stage and the proliferation index（PI） were increased（P〈0.05）, when the cells were stimulated by 5 %, 100% aloe gel. But there was no statistical difference in the cell cycle at the time point experiment（P〉0.05）. Compared with the control group the hydroxyproline in the cultural medium was increased（P〈0.05） ,when the cells were stimulated by aloe gel. The expression of procollagen type T in vitro was increased（P〈0. 05）after the cells stimulated by 10%aloe gel Conclusion Aloe gel can enhance the growth of human skin fibroblast and the collage synthesis.