单步溶胀聚合法制备单分散分子印迹聚合物微球

Preparation of Monodispersed Molecularly Imprinted Polymeric Microspheres by One-step Swelling Polymerization

用无皂乳液聚合法制得的微米级聚苯乙烯微球为种球,以N-甲基天门冬氨酸（NMA）为模板分子,通过单步溶胀聚合法在水相中制备了单分散分子印迹聚合物微球（MIPMs）。研究了影响种球溶胀的因素,并对MIPMs的结构进行了表征。实验表明,单步溶胀聚合法能够制得粒径2～3μm、单分散性好的MIPMs,且具有较好的特异吸附性能。Scatchard分析表明,MIPMs在识别NMA过程中存在两类结合位点,计算得到高亲和力的结合位点的离解常数KD1和最大表观结合常数Bmax1分别为0.223 mmol/L和2.12μmol/g,低亲和力的结合位点的离解常数KD2和最大表观结合常数Bmax2分别为5.34 mmol/L和36.42μmol/g。

Molecularly imprinted polymeric microspheres （MIPMs） were prepared by one-step swelling polymerization method in aqueous system using micron-polystyrene microspheres prepared by emulsiferfree polymerization as seeds and using N-methylasparagic acid （NMA） as cyclostyle molecules. Good monodispersed MIPMs were prepared with particle size in the range of 2 - 3 μm. Scatchard analysis suggested the MIPMs recognizing NMA with two classes of binding sites. The calculated dissociation constant KD1 and apparent maximum number Bmax1 of binding sites with high affinity were 0. 223 mmol/ L and 2. 12 μmol/g respectively, while KD2 and Bmax2 of binding sites with low affinity were 5.34 mmol/ L and 36.42 μmol/g.