人类嗜T淋巴细胞病毒I型的PCR检测方法研究

STUDY ON THE PCR FOR THE HUMAN T-CELL LYMPHOTROPIC VIRUS TYPE I DETECTED BY PCR

[目的]建立人类嗜T淋巴细胞病毒I型(HTLV-I)PCR检测方法。[方法]采集成人T淋巴细胞白血病(ALT)病人全血,分离外周血单核细胞(PBMCs),并与健康人PBMCs共培养分离HTLV-I;针对HTLV-I的gag和tax基因设计引物,进行PCR扩增,对扩增产物进行琼脂糖凝胶电泳检测HTLV-I。[结果]凝胶电泳检测到HTLV-I前病毒DNA的gag和tax片断。[结论]PCR法可快速、准确检测嗜人T细胞白血病病毒。

[Objective] To establish the polymerase chain reaction （PCR） method for detection of Human T-cell Lymphotropic virus type I （HTLV-I）. [Methods] PBMCs from peripheral blood specimens of patients with ATL were collected and cultured together With peripheral blood monocytes （PBMCs） donated by uninfected healthy donors to isolate HTLV-I; The primers regarding gag and tax of HTLV-I were designed to amplify with PCR, HTLV-I in the amplified products was detected by agamse gel electrophoresis. The DNA was amplified by PCR using primers gag and tax. The amplified products were detected with agarose gel electrophoresis. [ Results] The gag and tax section in DNA of HTLV-I provirus could detected by agarose gel electrophoresis. [Conclusion] PCR can rapidly and accurately detect the HTLV-I.