摘要
[目的]探讨微卫星DNA序列不稳定性(MSI)表达水平及3p24位点杂合缺失与食管癌发生的关系。[方法]应用聚合酶链反应(PCR)和变性聚丙烯酰胺凝胶电泳技术,对35例人食管癌中MSI表达情况进行研究;同时利用PCR-RFLP法检测其3p24位点杂合缺失情况,并对该片段进行克隆。[结果]食管癌MSI多发生在3p位点,在35例食管癌患者中共检出8例杂合缺失。[结论]食管癌在3p染色体位点存在微卫星不稳定现象,3p24突变可能与食管癌的发生有一定相关性。MSI和3p24位点杂合缺失也许可作为临床筛查恶性肿瘤高危人群的分子手段,具有潜在的应用前景。
[Objective] To explore the the expression of microsatellite instability (MSI) and the relationship between lack of miscellaneous on chromosome 3p24 and esophageal carcinoma. [ Methods] The expression of MSI among 35 patients with esophageal cancer was studies by using polymerase chain reaction (PCR) and denaturalized polyacrylamide gel electrophoresis technique. At tile same time, the status of lack of miscellaneous on 3p24 was detected by PCR-RFLP, then this fragment was cloned. [ Results] The MSI was often occurred at 3p site, 8 cases lack of miscellaneous were found in 35 patients with esophageal cancer. [ Conclusion] MSI can be detected at 3p site among patients with esophageal carcinoma, mutation of 3p24 have a certain correlation with esophageal cancer. Lack of miscellaneous on chromosome 3p24 and MSI could be a promising molecular index for screening malignancy among high risk population.
出处
《现代预防医学》
CAS
北大核心
2008年第12期2314-2316,共3页
Modern Preventive Medicine
关键词
食管癌
不稳定性
肿瘤
Esophageal carcinoma
Microsatellite instability
Tumor