积雪草总三萜酸及其主要成分的抗抑郁活性研究

Antidepressant activity of Centalla Asiatic Triterpenic acid and its main constituents

目的:探讨积雪草总三萜酸和其主要成分积雪草酸、羟基积雪草酸的抗抑郁作用。方法:采用小鼠强迫游泳实验、小鼠悬尾实验、开野实验和拮抗利血平所致的小鼠眼睑下垂实验,分别以小鼠不动时间、自主活动数和拮抗率作为评价指标。结果:在强迫游泳实验中积雪草总三萜酸、积雪草酸、羟基积雪草酸60 mg/kg、120 mg/kg剂量均能显著缩短小鼠不动时间,在悬尾实验中30 mg/kg、60 mg/kg、120 mg/kg剂量均能显著缩短小鼠不动时间,开野实验结果表明给药后小鼠的自主活动无明显变化,在利血平拮抗实验中积雪草总三萜酸、积雪草酸、羟基积雪草酸均可减少小鼠眼睑下垂。结论:积雪草总三萜酸、积雪草酸、羟基积雪草酸有抗抑郁作用。

Objective： To investigate the antidepressant activity of Centalla asiatic triterpenic acid （ CATA）, and its main comstituerts asiatic acid （AA） and madecassic acid （ MA ）. Methods： The forced swimming test （FST） , tail suspension test （TST） , open field activity（OFA） test and reserpine-induced ptosis test were carried out to examine the antidepressant activity in mice. Mice immobility duration in FST and TST, spontaneous activity frequency in OFA, and ptosis in reserpine antagonistic test were observed. Results： CATA, AA and MA could significantly shorten mice immobility time in FST at the dosages of 60 and 120 mg/kg and could markedly shorten mice immobility time in TST at the dosages of 30, 60 and 120 mg/kg. They could not reduce mice spontaneous activity in OFA. CATA, AA and MA could reduce ptosis mice in reserpine antagonistic test. Conclusion ： CATA, AA and MA exert an anti- depressant activity in animal models.